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Book Chapters

Wilkie, MP, Johnson, NS, Docker, MF. 2022. Chapter 10: Invasive species control and management: The sea lamprey story.  Pages 489-579 in Conservation Physiology for the Anthropocene - Fish Physiology series. Volume 39B. N Fangue, SJ Cooke, AP Farrell, CJ Brauner, EJ Eliason (editors). Elsevier-Academic Press, Cambridge, MA. 

Zydlewski, J. and M.P. Wilkie. 2013. Freshwater to seawater transitions in migratory fishes. Chapter 6 Pages 253-326 in Fish Physiology, Volume 32, Euryhaline Fishes. S.D. McCormick, A.P. Farrell; C.J. Brauner (volume editors), Academic Press. 

Wilkie, M.P. 2011. The physiology of lampreys I: Energetics and development. Chapter 252 in The Encyclopedia of Fish Physiology: From Genome to Environment. A.P. Farrell (editor-in-chief), Elsevier Inc. 

Wilkie, M.P. 2011. The physiology of lampreys II: Environmental physiology. Chapter 279 in The Encyclopedia of Fish Physiology: From Genome to Environment. A.P. Farrell (editor-in-chief), Elsevier Inc. 

Peer-Reviewed Publications

Borowiec BG, McDonald AE, Wilkie MP. 2023. Upstream migrant sea lamprey (Petromyzon marinus) show signs of 2 increasing oxidative stress but maintain aerobic capacity with age. Comparative Physiology and Biochemistry –Part A 285 (2023) 111503.  DOI. 10.1016/j.cbpa.2023.111503. 

Lawrence MJ, Grayson P, Jeffrey JD, Docker MF, Garroway CJ, Wilson JM, Manzon RG, Wilkie MP, Jeffries KM. 2023.  Differences in the transcriptome response in the gills of sea lamprey acutely exposed to 3-trifluoromethyl-4-nitrophenol (TFM), niclosamide or a TFM:niclosamide mixture. Comparative Biochemistry and Physiology- Part D: Genomics and Proteomics 48 (2023) 101122. 

Lawrence MJ, Grayson P, Jeffrey JD, Docker MF, Garroway CJ, Wilson JM, Manzon RG, Wilkie MP, Jeffries KM. 2023. Transcriptomic impacts and potential routes of detoxification in a lampricide-tolerant teleost exposed to TFM and niclosamide. Comparative Biochemistry and Physiology – Part D: Genomics and Proteomics. 46 (2023) 101074. 

Birceanu O*, Ferreira P, Neal J, Sunga J, Anthony S, Davidson SM, Edwards SL, Wilson JM, Youson JY, Vijayan MM, Wilkie, MP. 2022. Divergent pathways of ammonia and urea production and excretion during the life cycle of the sea lamprey. Physiological and Biochemical Zoology 95, 551-567. DOI10.1086/721606. 

Lawrence MJ, Grayson P, Jeffrey JD, Docker MF, Garroway CJ, Wilson JM, Manzon RG, Wilkie MP, Jeffries KM. 2022. Variation in the transcriptome response and detoxification gene diversity drives pesticide tolerance in fishes. Environmental Science and Technology 56, 12137-12147. DOI10.1021/acs.est.2c0082.

Clifford AM, Wilkie MP, Edwards SL, Tresguerres M, Goss GG. 2022. Dining on the dead in the deep: Active NH4+ excretion via Na+/H+(NH4+) exchange in the highly ammonia tolerant Pacific hagfish, Eptatretus stoutii. Acta Physiologica. e13845. DOI10.1111/apha.13845. 

Borowiec B, Birceanu O, Wilson JM, McDonald A, Wilkie, MP. 2022. Niclosamide is a much more potent inhibitor of mitochondrial respiration than TFM in invasive sea lamprey (Petromyzon marinus). Environmental Science and Technology. 56, 4970-4979. Journal Impact Factor: 12.154.

Ionescu, R.A., Mitrovic, D., Wilkie, M.P. 2022a. Reversible disruptions to energy supply and acid-base balance in larval sea lamprey exposed to the pesticide: Niclosamide (2 ',5-dichloro-4 '-nitrosalicylanilide). Aquatic Toxicology 242, 106006. DOI: 10.1016/j.aquatox.2021.106006.

Ionescu, R.A., Mitrovic, D., Wilkie, M.P. 2022b. Disturbances to energy metabolism in juvenile lake sturgeon (Acipenser fulvescens) following exposure to niclosamide. Exotoxicology and Environmental Safety 229, 112969. DOI: 10.1016/j.ecoenv.2021.112969.

Lantz, S.R., Adair, R.A., Amberg, J.J., Bergstedt, R.A., Boogaard, M.A., Bussy, U., Docker, M.F., Dunlop, E.S., Gonzalez, A., Hubert, T., Siefkes, M.J., Sullivan, W.P., Whyard, S., Wilkie, M.P., Young, B., Muir, A.M. 2022. Next Generation Lampricides: A three-stage process to develop improved control tools for invasive sea lamprey. Canadian Journal of Fisheries and Aquatic Sciences 79, 692-702.

Hepditch, S.L.J., Birceanu, O., Wilkie, M.P. 2021. A toxic unit and additive index approach to understanding the interactions of two piscicides, 3-trifluoromethyl-4-nitrophenol and niclosamide, in rainbow trout. Environmental Toxicology and Chemistry 40, 1419-1430. DOI: 10.1002/etc.4994.

Ionescu AR, Hepdicth SLJ, Wilkie, M.P. 2021. The lampricide 3-trifluoromethyl-4-nitrophenol causes temporarymetabolic disturbances in juvenile lake sturgeon (Acipenser fulvescens): implications for sea lamprey control and fish conservation. Conservation Physiology 9. WOS:000722546500001 DOI: 10.1093/conphys/coab069.

Lawrence, M.J., Mitrovic, D., Foubister, D., Bragg, L.M., Sutherby, J., Docker, M.F., Servos, M.R., Wilkie, M.P., Jeffries, K.M., 2021. Contrasting physiological responses between invasive sea lamprey and non-target bluegill in response to acute lampricide exposure. Aquatic Toxicology 237, 105848. DOI: 10.1016/j.aquatox.2021.105848.

McLaughlin, R., Adams, J.V., Almeida, P.R., Barber, J., Burkett, D.P., Docker, M.F., Johnson, N.S., Moser, M.L., Muir, A.M., Pereira, D.L., Siefkes, M.J., Steeves, T.B., Wilkie, M.P. 2021. Foreword: Control and conservation of lampreys beyond 2020-Proceedings from the 3rd Sea Lamprey International Symposium (SLIS III). Journal of Great Lakes Research 47, S1-S10. DOI: 10.1016/j.jglr.2021.11.00.

Birceanu, O., Tessier, L.R., Huerta, B., Li, W.M., McDonald, A., Wilkie, M.P. 2021. At the intersection between toxicology and physiology: What we have learned about sea lampreys and bony fish physiology from studying the mode of action of lampricides. Journal of Great Lakes Research 47 (suppl. 1), S673-S689. DOI: 10.1016/j.jglr.2021.07.007.

Borowiec, B.G., Docker, M.F., Johnson, N.S., Moser, M.L., Zielinksi, B., Wilkie, M.P. 2021. Exploiting the physiology of lampreys to refine methods of control and conservation. Journal of Great Lakes Research 2021 Vol. 47 Pages S723-S741. DOI: 10.1016/j.jglr.2021.10.015.

Hlina, B.L., Birceanu, O., Robinson, C.S., Dhiyebi, H., Wilkie, M.P. 2021. The relationship between thermal physiology and lampricide sensitivity in larval sea lamprey (Petromyzon marinus). Journal of Great Lakes Research 47 (suppl. 1), S272-S284. DOI: 10.1016/j.jglr.2021.10.002. 

Wilkie, M.P., Tessier, L.R., Boogaard, M., O'Connor, L., Birceanu, O., Steeves, T.B., Sullivan, W.P., 2021. Lampricide bioavailability and toxicity to invasive sea lamprey and non-target fishes: The importance of alkalinity, pH, and the gill microenvironment. Journal of Great Lakes Research 47 (suppl. 1), S407-S420. DOI: 10.1016/j.jglr.2021.09.005 (90 %)


Sunga, J, Wilson, JM, Wilkie, MP. (2020). Re-organization of the gills of metamorphosing sea lamprey (Petromyzon marinus): Functional linkages between osmoregulation and nitrogenous waste excretion. Journal of Comparative Physiology - B. 190: 701-715. DOI: 10.1007/s00360-020-01305-1

Kolosov D, Bui P, Wilkie MP, Kelly SP. (2020). Claudins of sea lamprey – tissue expression and salinity-induced alterations in transcript abundance in larval and adult life stages. Journal of Fish Biology 96, 768-781. DOI: 10.1111/jfb.14274.

Wilkie, MP, Hubert, TD, Boogaard, MA, Birceanu, O. (2019). Control of invasive sea lampreys using the piscicides TFM and niclosamide: Toxicology, successes & future prospects. Aquatic Toxicology 211: 235-252.

Lennox, RJ, Bravener, GA, Lin, H-Y, Madenjian CP, Muir, AM, Remucal, CK, Robinson KF, Rous, AM, Siefkes, MJ, Wilkie, MP, Zielinski, DP, Cooke, SJ. (2020). Potential changes to the biology and challenges to the management of invasive sea lamprey Petromyzon marinus in the Laurentian Great Lakes due to climate change. Global Change Biology 2020.00, 1-20. DOI: 10.1111/gcb.14957.

Muhametsafina, M, Birceanu, O, Hlina, BL, Tessier, LR, Wilkie, MP. (2019). Warmer Waters Increase the Larval Sea Lamprey's (Petromyzon marinus) Tolerance to the Lampricide 3-trifluoromethyl-4nitrophenol (TFM). Journal of Great Lakes Research 45: 921-933.


Hepditch, LJ, Tessier, LR, Wilson, JM, Birceanu, O, O'Connor, L, Wilkie, MP. (2019). Mitigation of lampricide toxicity to juvenile lake sturgeon: the importance of water alkalinity and life stage. Conservation Physiology 7,17 pp. DOI: 10.1093/conphys/coz089.


Vo, N.T.K, Moore, L.C., Spiteri, K.W., Hanner, R, Wilkie, M.P., and DeWitte-Orr, S.J. 2018. Assessing off-target cytotoxicity of the field lampricide 3-trifluoromethyl-4-nitrophenol using novel lake sturgeon cell lines. Ecotoxicology and Environmental Safety 162: 536-545. DOI: 10.1016/j.ecoenv.2018.07.017

Birceanu, O, and Wilkie, M.P. 2018. Post-exposure effects of the piscicide 3-trifluoromethyl-4-nitrophenol (TFM) on the stress response and liver metabolic capacity in rainbow trout (Oncorhynchus mykiss). PLOS One 13, Issue 7. DOI: 10.1371/journal.pone.0200782.

Tessier, L.R., Long, T.A.F., and Wilkie, M.P. 2018. Influence of body size, metabolic rate & life history stage on uptake & excretion of the lampricide 3-trifluoromethyl-4-nitrophenol (TFM) by invasive sea lampreys (Petromyzon marinus). Aquatic Toxicology 194: 27-36. DOI:10.1016/j.aquatox.2017.10.020.

Dunlop, E.S., McLaughlin, R., Adams, J.V., Jones, M., Birceanu, O., Christie, M.R., Criger, L.A., Hinderer, J.L.M., Hollingworth, R.M., Johnson, N.S., Lantz, S.R., Li, W.M., Miller, J., Morrison, B.J., Mota-Sanchez, D., Muir, A., Sepulveda, M.S., Steeves, T., Walter, L., Westman, E., Wirgin, I., and Wilkie, M.P. 2018. Rapid evolution meets invasive species control: the potential for pesticide resistance in sea lamprey. Canadian Journal of Fisheries and Aquatic Sciences 75:152-168. doi: 10.1139/cjfas-2017-0015. 

Kolosov, D., Phuong, B., Donini, A., Wilkie, M.P. and Kelly, S.P. 2017. A role for tight junction-associated MARVEL proteins in larval sea lamprey (Petromyzon marinus) osmoregulation. Journal of Experimental Biology 220: 3657-3670.  doi: 10.1242/jeb.161562. 

Clifford, A.M., A.M. Weinrauch, S.L. Edwards, M.P. Wilkie, and G.G. Goss. 2017. Flexible ammonia handling strategies using the skin and gill contribute to the high ammonia tolerance of the Pacific hagfish. American Journal of Physiology 313: R78-R90. doi: 10.1152/ajpregu.00351.2016.

Hlina, B.L., L.R. Tessier, and M.P. Wilkie. 2017. Effects of water pH on the uptake & elimination of a piscicide, 3-trifluoromethyl-4-nitrophenol (TFM), by larval sea lampreys. Comparative Biochemistry and Physiology-Part C. 200: 9-16. doi: 10.1016/j.cbpc.2017.05.005. 

Wilkie, M.P., A.M. Clifford, S.L. Edwards, and G.G. Goss. 2017. Wide scope for nitrogen catabolism in the foraging Pacific hagfish. Marine Biology 164. Article Number 126. doi. 10.1007/s00227-017-3148-3.

Blair, S.D., Wilkie, M.P., and Edwards, S.L. 2017. Rh glycoprotein immunoreactivity in the skin and its role in extrabranchial ammonia excretion by the sea lamprey (Petromyzon marinus) in fresh water. Canadian Journal of Zoology 95: 95-105. DOI: 10.1139/cjz-2016-0120.

Hossein-Javaheri, N., Wilkie, M.P., Lado, W.E., and Buck, L.T. 2017. Stellate and pyramidal neurons in goldfish telencephalon respond differently to anoxia and GABA receptor inhibition. Journal of Experimental Biology 220: 695-704. doi: 10.1242/jeb.146605.

Lisser, D.F.J, Lister, Z.M., Pham-ho P.Q.H., Scott, G.R., Wilkie, M.P. 2017. Relationship between oxidative stress and brain swelling in goldfish (Carassius auratus) exposed to high environmental ammonia. American Journal of Physiology - Regulatory, Integrative and Comparative Physiology. 312(1):R114-R124.

Clemow, Y.H., and Wilkie, M.P. 2015. Effects of Pb plus Cd mixtures on toxicity, and internal electrolyte and osmotic balance in the rainbow trout (Oncorhynchus mykiss). Aquatic Toxicology. 161:76-84.

Henry, M., O. Birceanu, G.B. McClelland, A.M. Clifford, Y.S. Wang, M.P. Wilkie. 2015. Life-stage dependent responses to the lampricide 3-trifluoromethyl-4-nitrophenol (TFM) provide insight into glucose homeostasis and metabolism in sea lamprey (Petromyzon marinus). Comparative Biochemistry and Physiology, Part C: Toxicology and Pharmacology. 169: 35-45. 


Clifford, A.M, G.G. Goss, M.P. Wilkie. 2015. Adaptations of a deep sea scavenger: Extreme ammonia tolerance and active NH4+ excretion by the Pacific hagfish (Eptatretus stoutii). Comparative Biochemistry and Physiology, Part A: Molecular and Integrative Physiology. 182:64-74. 


Wilkie, M.P., J.A.W. Stecyk, C.S. Couturier, S. Sidhu, G.K. Sandvik, and G.E. Nilsson. 2015. Reversible brain swelling in crucian carp (Carassius carassius) and goldfish (Carassius auratus) in response to high external ammonia and anoxia. Comparative Biochemistry and Physiology, Part A: Molecular and Integrative Physiology. 184:65-75. 


Birceanu O., L.A. Sorensen, M. Henry, G.B. McClelland, Y.X.S. Wang, M.P. Wilkie. 2014. The effects of the lampricide 3-trifluoromethy1-4-nitrophenol (TFM) on fuel stores and ion balance in a non-target fish, the rainbow trout (Oncorhynchus mykiss). Comparative Biochemistry and Physiology, Part C: Toxicology and Pharmacology. 160:30-41.


Havixbeck J.J., A.M. Rieger, M.E. Wong, M.P. Wilkie, D.R. Barreda. 2014. Evolutionary conservation of divergent pro-inflammatory and homeostatic responses in lamprey phagocytes. PLOS ONE. 9(1): e86255.


Binder TR, McDonald DG, Wilkie MP. 2013. Reduced dermal photosensitivity in juvenile sea lampreys (Petromyzon marinus) reflects life-history-dependent changes in habitat and behaviour. Canadian Journal of Zoology. 91:635-639.


Clifford, A.M., M. Henry, A. Smits, D.G. McDonald, R. Bergstedt, M.P. Wilkie.  2012. Recovery of larval sea lampreys from short-term exposure to the pesticide, 3-trifluoromethyl-4-nitrophenol (TFM): Implications for sea lamprey control in the Great Lakes. Transactions of the American Fisheries Society. 141:1697-1710.


Winter, A.R., R.C. Playle, D.G. Dixon, U. Borgmann, and M.P. Wilkie. 2012. Interactions of Pb and Cd mixtures in the presence or absence of natural organic matter with the fish gill. Ecotoxicology and Environmental Safety. 83:16-24. 


Wilkie, M.P., M. Pamenter, S. Duquette, H. Dhiyebi, G. Skelton, N. Sangha, and L.T. Buck. 2011. The relationship between NMDA receptor function and the high ammonia tolerance of anoxia-tolerant goldfish (Carassius auratus).  Journal of Experimental Biology. 214:4107-4120.


Birceanu, O., G.B. McClelland, Y.S. Wang, J.C.L. Brown, and M.P. Wilkie. 2011. The lampricide 3-trifluoromethyl-4-nitrophenol (TFM) uncouples mitochondrial oxidative phosphorylation in both sea lamprey (Petromyzon marinus) and TFM-tolerant rainbow trout (Oncorhynchus mykiss). Comparative Biochemistry and Physiology, Part C:Toxicology and Pharmacology. 153:342-349. 


Gheorghiu, C., D.S. Smith, H.A. Al-Reasi, J.C. McGeer, and M.P. Wilkie. 2010. Influence of natural organic matter (NOM) quality on Cu-gill binding in the rainbow trout (Oncorhynchus mykiss).  Aquatic Toxicology. 97:343–352. 


Gillis, P.L, J.C. McGeer, G.L. Mackie, M.P. Wilkie, and J.D.Ackerman. 2010. The effect of natural dissolved organic carbon on the sensitivity of larval freshwater mussels (glochidia) to acute copper exposure. Environmental Toxicology and Chemistry. 29:2519–2528. 


Gheorghiu, C., J. Hanna, J.W. Smith, D. S. Smith, and M.P. Wilkie. 2010. Encapsulation  and migration of PIT tags implanted in brown trout (Salmo trutta L.). Aquaculture. 298: 350-353.


Birceanu, O., G.B. McClelland, Y.S. Wang, and M.P. Wilkie. 2009. The mechanism of lampricide, 3-trifluoromethyl-4-nitrophenol (TFM), toxicity in larval sea lampreys (Petromyzon marinus): A failure of ATP supply to match ATP demand.  Aquatic Toxicology. 94 265-274. 

Newby, N.C., M.P. Wilkie, and E.D. Stevens. 2009. Morphine uptake, disposition, and analgesic efficacy in the common goldfish (Carassius auratus).  Canadian Journal of Zoology. 87:388-399. 

Weihrauch, D., M.P. Wilkie, and P.J. Walsh. 2009. Ammonia and urea transporters in gills of fish and aquatic crustaceans. Journal of Experimental Biology. 212:1716-1730.

Birceanu, O., M.J. Chowdhury, P.L. Gillis, J.C. McGeer, C.M. Wood, and M.P. Wilkie. 2008. Modes of metal toxicity and impaired branchial ionoregulation in rainbow trout exposed to mixtures of Pb and Cd in soft water. Aquatic Toxicology. 89: 221-231. 

Wilkie, M.P., M.E., Pamenter, S. Alkabie, D. Carapic, D. S.-H. Shin, and L.T. Buck. 2008. Evidence of anoxia-induced channel arrest in the brain of the goldfish (Carassius auratus).  Comparative Biochemistry and Physiology Part A. 148C: 355-362. 


Wilkie, M.P., J.A. Holmes, and J.H. Youson. 2007. The lampricide, 3-trifluoromethyl-4-nitrophenol (TFM) interferes with intermediary metabolism and glucose homeostasis, but not ion balance in larval lampreys (Petromyzon marinus). Canadian Journal of Fisheries & Aquatic Sciences. 64:1174-1182.

Wilkie, M.P., T.P. Morgan, F. Galvez, R.W. Smith, M. Kajimura, Y.K. Ip, and C.M. Wood. 2007. The African lungfish (Protopterus dolloi): Ionoregulation and osmoregulation in a fish out of water. Physiological and Biochemical Zoology. 80:99-112.

Walsh, P.J., C.M. Veauvy, M.D. McDonald, M.E. Pamenter, L.T. Buck, and M.P. Wilkie. 2007. Piscine insights into comparisons of anoxia tolerance, ammonia toxicity, stroke and hepatic encephalopathy. Comparative Biochemistry and Physiology, Part A. 147:332-343.

Wilkie, M.P., J.F. Claude, A. Cockshutt, J.A. Holmes, Y.S. Wang, J.H. Youson and P.J. Walsh. 2006. Shifting patterns of nitrogenous waste excretion and amino acid catabolism capacity during the life cycle of the sea lamprey (Petromyzon marinus). Physiological and Biochemical Zoology. 79:885-898.

Shin, D.S., M.P. Wilkie, M. Pamenter, and L.T. Buck. 2005. Calcium and protein phosphatase 1 and 2A attenuate N-methyl-D aspartate receptor activity in the anoxic turtle cortex. Comparative Biochemistry and Physiology, Part A. 142:50-57.

Wilkie, M.P., S. Turnbull, J. Bird, Y. S. Wang, J.F. Claude, and J.H. Youson. 2004. Lamprey parasitism of sharks and teleosts: high capacity urea excretion in an extant vertebrate relic. Comparative Biochemistry and Physiology, Part A. 138:485-492.

Wilkie, M.P. 2002. Ammonia excretion and urea handling by fish gills: present understanding and future research challenges. Invited review article included in a special edition of the Journal of Experimental Zoology entitled “Anatomy and Physiology of the Fish Gill: A Prospective Approach.” David H. Evans and Kenneth R. Olson eds. 293:284-301.

Wilkie, M.P., P.G. Bradshaw, V. Joanis, J.F. Claude, and S.L. Swindell. 2001. Rapid metabolic recovery following vigorous exercise in burrow-dwelling larval sea lampreys (Petromyzon marinus). Physiological and Biochemical Zoology. 74:261-272.

Wilkie, M.P., P. Laurent, and C.M. Wood. 1999. The physiological basis for altered Na+ and Cl-movements across the gills of rainbow trout in highly alkaline (pH 9.5) water. Physiological and Biochemical Zoology. 72:360-368.

Wilkie, M.P., J. Couturier, and B.L. Tufts. 1998. Mechanisms of acid-base regulation in migrant sea lampreys (Petromyzon marinus) following exhaustive exercise. Journal of Experimental Biology. 201:1473-1482.

Wilkie, M.P. 1997. Mechanisms of ammonia excretion across fish gills. Invited Review.Comparative Biochemistry and Physiology. 118A:39-50.

Wilkie, M.P., M.A. Brobbel, L. Forsyth, K. Davidson and B.L. Tufts. 1997. The influence of temperature on the post-exercise physiology and survival of Atlantic salmon (Salmo salar). Canadian Journal of Fisheries and Aquatic Sciences. 54:503-511.

Wilkie, M.P., K. Davidson, M.A. Brobbel, J.D Kieffer, R.J. Booth, A. Bielak and B.L. Tufts. 1996. Physiology and survival of wild Atlantic salmon following angling in warm summer waters. Transactions of the American Fisheries Society. 125:572-580.

Wilkie, M.P. and C.M. Wood. 1995. Recovery from high pH exposure in the rainbow trout: white muscle ammonia storage, ammonia washout and the restoration of blood chemistry. Physiological Zoology. 68:379-401.


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